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15/03/2007 · Our kineticstructural results establish that the dendritic pathway does function in DRG growth cones that protrude veils under normal culture conditions. Further, the dendritic pathway biomarker, Arp2/3, was localized to the leading edge of protruding veils by both immunostaining and by expression of tagged Arp2/3 (both Myc and GFP). It is not clear why .
However the structural architecture of the muscle and the endplate integrity can be maintained for up to 1 year 69,89. After 2 years, irreversible muscle fibrosis has occurred along with muscle degeneration, leading to a permanent loss of functional muscle tissue. Sensory endorgans such as Paccinian corpuscles, Meissner corpuscles and Merkel cells can last up to 23 years, so .
cone periphery, so that they can probe intracellular space and respond to extracellular signals (13, 14). In contrast to advancing growth cones, injured axons form retraction bulbs, which contain disorganized MTs that fail to extend into the periphery (15, 16). Thus, reorganization of the MT network and subsequent formation of an advancing growth cone might promote axon growth .
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DRG neurons . DRG neurons were obtained as previously described [19,22,23].Briefly, Wistar rats 12 days old (P1P2) and 10 to 12 days old (P10P12) were saced by decapitation after being anesthetized by CO dissection, DRGs were incubated with mg/ml trypsin, 1 mg/ml collagenase and mg/ml DNase (all from SigmaAldrich, St. Louis, MO) in 5 ml Neurobasal medium (Life ...
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01/04/2003 · To quantify the motility of the growth cone, DRG neurons were infected with HSV coding for LIMK1YFP, LIMK1(D467A)YFP, or YFP as a control, and then the growth cone motility was monitored by timelapse video fluorescence microscopy. Individual images were digitized, and growth cone outlines between at 2 min time points were compared to calculate areas of new protrusion of the growth cone ...
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05/06/2020 · For growth cone collapse assays, DRG neurons were transfected at 1 day in vitro (DIV1) with Sema6AWTEGFPpCAG, Sema6AmutAEGFPpCAG, Sema6AmutBEGFPpCAG or Sema6AmutA+BEGFPpCAG mutant constructs or empty EGFPpCAG vector using lipofectamine 2000 for 45 min at 37°C. At DIV4, vehicle (medium) or purified Sema6AFc was added to the cultures at a .
01/04/2003 · To quantify the motility of the growth cone, DRG neurons were infected with HSV coding for LIMK1YFP, LIMK1(D467A)YFP, or YFP as a control, and then the growth cone motility was monitored by timelapse video fluorescence microscopy. Individual images were digitized, and growth cone outlines between at 2 min time points were compared to calculate areas of new protrusion of the growth cone ...
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